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Nat Methods. 2007 Jan;4(1):35-7. Epub 2006 Nov 19.

Sensitive and specific method for detecting G protein-coupled receptor mRNAs.

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  • 1Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institute of Health, Department of Health and Human Services, Bethesda, Maryland 20892, USA. arnehansen@nidcr.nih.gov

Abstract

G protein-coupled receptors (GPCRs) mediate effects of extracellular signaling molecules in all the body's cells. These receptors are encoded by scarce mRNAs; therefore, detecting their transcripts with conventional microarrays is difficult. We present a method based on multiplex PCR and array detection of amplicons to assay GPCR gene expression with as little as 1 mug of total RNA, and using it, we profiled three human bone marrow stromal cell (BMSC) lines.

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