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Methods Enzymol. 2006;416:159-82.

Lectin-resistant CHO glycosylation mutants.

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  • 1Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY, USA.

Abstract

Chinese hamster ovary (CHO) mutant cells with a wide variety of alterations in the glycosylation of proteins and lipids have been isolated by selection for resistance to the cytotoxicity of plant lectins. These CHO mutants have been used to characterize glycosylation pathways, to identify genes that code for glycosylation activities, to elucidate functional roles of glycans that mediate biological processes, and for glycosylation engineering. In this chapter, we briefly describe the available panel of lectin-resistant CHO mutants and summarize their glycan alterations and the biochemical and genetic bases of mutation.

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