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1: J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Apr 15;849(1-2):282-92. Epub 2006 Nov 20.Click here to read Links

Preparation of membrane proteins for analysis by two-dimensional gel electrophoresis.

Kashino Y, Harayama T, Pakrasi HB, Satoh K.

Department of Life Science, University of Hyogo, Ako-gun, Hyogo 678-1297, Japan. kashino@sci.u-hyogo.ac.jp

In order to separate hydrophobic membrane proteins, we have developed a novel two-dimensional electrophoresis system. For the iso-electric focusing, agarose was used as a supporting matrix and n-dodecyl-beta-D-maltopyranoside was used as a surfactant. In combination with a previously developed Tris/MES electrophoresis system in the second dimension, distinct spots were reproducibly detected from hydrophobic membrane proteins whose grand average hydropathicity (GRAVY) exceed 0.3. In contrast to the immobilized pH gradient system, c-type heme was also visualized in this system.

PMID: 17113836 [PubMed - indexed for MEDLINE]

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