Format

Send to:

Choose Destination
See comment in PubMed Commons below
Methods. 2006 Dec;40(4):312-7.

Purification and assay of the human INO80 and SRCAP chromatin remodeling complexes.

Author information

  • 1Stowers Institute for Medical Research, Kansas City, MO 64110, USA.

Abstract

Chromatin modifying and remodeling enzymes play critical roles in many aspects of chromosome biology including transcription, replication, recombination, and repair. Our laboratory recently identified and characterized two multisubunit human chromatin remodeling enzymes designated the INO80 and SRCAP complexes. Mechanistic studies revealed that the human INO80 complex catalyzes nucleosome sliding and the SRCAP complex catalyzes ATP-dependent exchange of histone H2A/H2B dimers containing the histone variant H2A.Z into nucleosomes. Here we describe methods for purification and assay of the INO80 and SRCAP chromatin remodeling complexes.

PMID:
17101442
[PubMed - indexed for MEDLINE]
PMCID:
PMC3092633
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Write to the Help Desk