Source
Department of Etiologic Biology, The Second Military Medical University, Shanghai 200433, China.
Abstract
OBJECTIVE:
To investigate the in vitro inhibition effect of the rabbit serum immunized with P. falciparum chimeric protein on the parasites by lactate dehydrogenase (LDH) method.
METHODS:
Sera of rabbits immunized with P. falciparum chimeric protein were used as test sample, with an initial parasitemia of (0.4 +/- 0.1)% and 2% hematocrit suspension. After 40-42 h of cultivation, LDH assay and a traditional microscopical method were applied to obtain the parasite inhibition rate and infection rate of erythrocytes.
RESULTS:
Sera of rabbits immunized with the protein showed a strong in vitro inhibition effect on P. falciparum. There was no significant difference of inhibition rates detected by the two methods (97% and 100% respectively) (P>0.05).
CONCLUSION:
The LDH method applied in the in vitro inhibition assay for P. falciparum seems simple, reliable and has generated a satisfactory result.