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J Biomed Opt. 2006 Sep-Oct;11(5):054032.

Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration.

Author information

  • 1Department of Physical Chemistry, Microgravity Research Center, Faculty of Applied Sciences, Universit√© Libre de Bruxelles, Brussels, Belgium 1050.

Abstract

Cancer cell motility and invasion are critical targets for anticancer therapeutics. Whereas in vitro models could be designed for rapid screening with a view to investigate these targets, careful consideration must be given to the construction of appropriate model systems. Most investigations focus on two-dimensional (2-D) assays despite the fact that increasing evidence suggests that migration across rigid and planar substrates fails to recapitulate in vivo behavior. In contrast, few systems enable three-dimensional (3-D) cell migration to be quantitatively analyzed. We previously developed a digital holographic microscope (DHM) working in transmission with a partially spatial coherence source. This configuration avoids the noise artifacts of laser illumination and makes possible the direct recording of information on the 3-D structure of samples consisting of multiple objects embedded in scattering media, such as cell cultures in matrix gels. The software driving our DHM system is equipped with a time-lapse ability that enables the 3-D trajectories of living cells to be reconstituted and quantitatively analyzed.

PMID:
17092181
[PubMed - indexed for MEDLINE]
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