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Biotechnol Lett. 2007 Jan;29(1):137-40. Epub 2006 Nov 8.

Purification of a trypsin inhibitor from sweet potato in an aqueous two phase system.

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  • 1Department of Bioengineering, Tatung University, Taipei, Taiwan.


Trypsin inhibitor from sweet potato was extracted and purified in a single step using an aqueous two-phase system of polyethylene glycol 6000 (11% w/v), phosphate (16.5% w/v), KCl (9% w/v) and at pH 6. Purity of the trypsin inhibitor was enhanced 3.7-fold, and the recovery was 95%. The purified trypsin inhibitor showed one visible band, and the molecular size was 23 kDa by SDS-PAGE.

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