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Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17137-42. Epub 2006 Nov 6.

Adaptive wavefront correction in two-photon microscopy using coherence-gated wavefront sensing.

Author information

  • 1Department of Biomedical Optics, Max-Planck Institute for Medical Research, Jahnstrasse 29, D-69120 Heidelberg, Germany. markus.rueckel@mpimf-heidelberg.mpg.de

Abstract

The image quality of a two-photon microscope is often degraded by wavefront aberrations induced by the specimen. We demonstrate here that resolution and signal size in two-photon microcopy can be substantially improved, even in living biological specimens, by adaptive wavefront correction based on sensing the wavefront of coherence-gated backscattered light (coherence-gated wavefront sensing, CGWS) and wavefront control by a deformable mirror. A nearly diffraction-limited focus can be restored even for strong aberrations. CGWS-based wavefront correction should be applicable to samples with a wide range of scattering properties and it should be possible to perform real-time pixel-by-pixel correction even at fast scan speeds.

PMID:
17088565
[PubMed - indexed for MEDLINE]
PMCID:
PMC1634839
Free PMC Article

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