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J Bacteriol. 1991 May;173(10):3134-7.

Evidence for energy-dependent transposition of core lipopolysaccharide across the inner membrane of Salmonella typhimurium.

Author information

  • 1Department of Microbiology, University of Connecticut Health Center, Farmington 06030.

Abstract

The uncoupler 2,4-dinitrophenol blocks the final step of lipopolysaccharide assembly--transfer of O antigen from undecaprenyl pyrophosphate to core lipopolysaccharide--in intact Salmonella typhimurium but not in isolated membrane fractions. The O-antigen ligase enzyme is not inhibited by dinitrophenol in vitro, and core lipopolysaccharide synthesized in the presence of uncoupler in vivo is functional as acceptor of O antigen in vitro. The evidence strongly suggests that maintenance of proton motive force is required for transmembrane transposition of core lipopolysaccharide to the active site of O-antigen ligase at the periplasmic face of the inner membrane.

PMID:
1708762
[PubMed - indexed for MEDLINE]
PMCID:
PMC207907
Free PMC Article
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