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Allergy. 2006 Dec;61(12):1473-9.

Release of prostaglandin D2 and leukotriene C4 in response to hyperosmolar stimulation of mast cells.

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  • 1Division of Physiology, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.

Abstract

BACKGROUND:

Mannitol-induced bronchoconstriction in subjects with exercise-induced asthma is associated with increased urinary excretion of 9alpha, 11beta-PGF(2), a metabolite of prostaglandin D(2) (PGD(2)) serving as a mast cell marker. It has however been questioned whether or not human mast cells release PGD(2) and leukotriene C(4) (LTC(4)) after osmotic challenge with mannitol in vitro.

METHODS:

Cord blood-derived human mast cells were stimulated osmotically, immunologically or with a combination of both. Supernatants were analysed for PGD(2), LTC(4) and histamine contents with enzyme immunoassays.

RESULTS:

Significant release of de novo synthesized eicosanoids, predominantly PGD(2) [12 (8.8, 14) pmol/10(6)cells; median (25th, 75th percentile) but also LTC(4) (0.1 (0.08, 0.15) pmol/10(6) cells] were found in mast cells in vitro in response to 0.7 M mannitol stimulation. A massive release of histamine [70 (5.3)% of total; mean (SEM)] was also found. There were no correlations between the levels of released mediators after mannitol stimulation. In contrast, there was a correlation between release of PGD(2) and LTC(4), following immunological stimulation.

CONCLUSION:

The findings support that hyperosmolar challenge activates mast cells, but different than antigen stimulation.

[PubMed - indexed for MEDLINE]
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