A) Entropy plot for avian versus human influenza viruses for NP amino acid residues. In each aligned position, we have a consensus residue for 95 avian strains displayed on top and a consensus residue for 306 human strains at the bottom. Completely conserved amino acid positions are filled with white; less conserved amino acids are filled in various gray shadings. Positions in which 1 single residue dominates >90%, <90% but >75%, and <75% are labeled with red, yellow, and green letters, respectively. Yellow rectangles indicate that both human and avian viruses are completely conserved to the same residue; magenta rectangles indicate that avian and human viruses are each completely conserved to a different residue. B) Entropy plots for the entire influenza A viral genome. Each lane displays entropy value distributions of aligned protein sequences for 1 of the 11 viral proteins; the upper half represents 95 avian strains, and the bottom half represents 306 human strains. (PB1-F2 contains fewer strains, as described in Discussion.) Positions completely conserved to a single residue are shown in a white band, while less conserved ones are shown in various gray shadings. The average entropy for the entire segment is shown to the right of these lanes. Entropy values are zero when residues are completely conserved; more negative values indicate more diversity. Alignment size for each protein from top to bottom is 759, 757, 90, 716, 591, 498, 480, 252, 97, 230, and 121.

Entropy plot for all 11 influenza proteins for human (top) versus avian (bottom). In each aligned position, we have a consensus residue for 95 avian strains displayed on top, and a consensus residue for 306 human strains at the bottom. Completely conserved amino acid positions are filled with white, while less conserved amino acids are filled in various gray shadings. Positions where one single residue dominates over 90%, less than 90% but greater than 75%, and less than 75% are labeled with red, yellow, and green letters, respectively. Yellow rectangles indicate that both human and avian flu are completely conserved to the same residue, while rectangles in magenta indicate that avian and human flu each completely conserves to a different residue Additional plots for HA, NA, NS1 and NS2, for using different counts of human or avian strains are detailed as individual captions to these plots. Adobe Acrobat PDF available at http://wwwnc.cdc.gov/eid/pdfs/06-0276-FA1.pdf (21 pages).

Histograms on comparing 306 human versus 95 avian influenza A viruses, based on nucleotide pairwise sequence identities. Vertical axis shows the count for pairs of sequences with specific percent identity (rounded to integer). Red bars represent frequencies for 'homo' pairs – sequences of the same host species (human to human, or avian to avian); blue bars represent frequencies for 'hetero' pairs – pairs that cross host species (human to avian, or avian to human). Adobe Acrobat PDF available at http://wwwnc.cdc.gov/eid/pdfs/06-0276-FA2.pdf (6 pages).

Histograms compare 43 avian allele A viruses and 306 human viruses (panels A and C), and 52 avian allele B viruses and 306 human viruses (panels B and D), based on their NS1 and NS2 genomic segments. Vertical axis shows the count for pairs of sequences with specific percent identity (rounded to integer). Red bars represent frequencies for 'homo' pairs – sequences of the same host species (human to human, or avian to avian); blue bars represent frequencies for 'hetero' pairs – pairs that cross host species (human to avian, or avian to human). Adobe Acrobat PDF available at http://wwwnc.cdc.gov/eid/pdfs/06-0276-FA3.pdf (5 pages).