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J Biotechnol. 2007 Feb 1;128(2):403-7. Epub 2006 Oct 27.

Deproteinization of gellan gum produced by Sphingomonas paucimobilis ATCC 31461.

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  • 1State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.


Deproteinization is a technical bottleneck in the purification of viscous water-soluble polysaccharides. The aim of this work is to provide an appropriate approach to deproteinize crude gellan gum. Several methods of deproteinization were investigated, including Sevag method, alkaline protease, papain and neutral protease. The results revealed that Sevag method had high deproteinization efficiency (87.9%), but it showed dissatisfactory recovery efficiency of gellan gum (28.6%), which made it less advisable in industrial applications. The deproteinization by alkaline protease was demonstrated in this work for the first time, indicating alkaline protease was preferred in the deproteinization of crude gellan gum with high polysaccharide recovery (89.3%) and high deproteinization efficiency (86.4%).

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