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Zhonghua Gan Zang Bing Za Zhi. 2006 Oct;14(10):735-7.

[A study on the relativity between La protein and the stability of HBV mRNA and the expression of HBV protein].

[Article in Chinese]

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  • 1Clinical Laboratory, Oriental Hospital, Shanghai 201200, China.

Abstract

OBJECTIVE:

To study the relativity between La protein and the stability of HBV mRNA and the expression of HBV protein.

METHODS:

Four specific siRNAs were obtained by transcription in vitro. After transfection with the siRNAs into HepG2.2.15 cells for 3 days, the inhibitive effects of La protein were analyzed by Western blot; the content changes of HBsAg, HBeAg and HBV-DNA were detected by ECL and RT-PCR.

RESULTS:

In comparison to normal cells, La protein was less in the cells. There was less La protein in the cells trans-infected with siRNAs. HBsAg, the HBeAg and HBV-DNA secreted by the cells transfected with siRNA were also less than that in the normal cells.

CONCLUSION:

There is a correlation between La protein and HBV mRNA and the expression of HBV protein.

PMID:
17064465
[PubMed - indexed for MEDLINE]
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