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Antimicrob Agents Chemother. 2007 Jan;51(1):394-6. Epub 2006 Oct 23.

Effects of inoculum size and incubation time on broth microdilution susceptibility testing of lactic acid bacteria.

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  • 1Microbiology Division, National Food Administration, Box 622, SE-751 26 Uppsala, Sweden. mia.egervarn@slv.se

Abstract

Inoculum size and incubation time were varied during broth microdilution testing of the susceptibilities of 35 strains of lactic acid bacteria to six antibiotics. An increase in either parameter resulted in elevated MICs for all species. An inoculum of 3 x 10(5) CFU/ml is recommended to assess the antibiotic susceptibilities of these bacteria by using broth microdilution.

PMID:
17060527
[PubMed - indexed for MEDLINE]
PMCID:
PMC1797673
Free PMC Article
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