Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1358-68. Epub 2006 Oct 18.

Automated detection and centring of cryocooled protein crystals.

Source

European Molecular Biology Laboratory, c/o DESY, Notkestrasse 85, 22603 Hamburg, Germany.

Abstract

A novel method is presented for the automated recognition of cryocooled macromolecular crystals. The method uses several texture-based image-processing algorithms for automated crystal centring, which are able to cope with a variety of crystal morphologies and illumination conditions. The results combined from different algorithms, together with their estimated standard uncertainties, provide a robust determination of the crystal location and allow an internal assessment of their reliability. The method was coded within the software XREC and showed a good performance on 104 sets of images from various beamlines.

PMID:: 17057339; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

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Proteins

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