Exp Hematol. 2006 Nov;34(11):1596-601.

Multipotent adult progenitor cell isolation and culture procedures.

Breyer A, Estharabadi N, Oki M, Ulloa F, Nelson-Holte M, Lien L, Jiang Y.

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University of Minnesota, Stem Cell Institute, Minneapolis, MN 55455, USA.

Abstract

We describe methods for isolation of multipotent adult progenitor cells (MAPCs) from newborn to 6-week-old mice and rats. The maintenance of these cells, including their culture, media formulas, and quality control procedures, are also explained. Additionally, ways to identify MAPCs including their phenotype and morphology are discussed.

PMID:: 17046581; [PubMed - indexed for MEDLINE]

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