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J Infect Dis. 2006 Nov 1;194(9):1258-66. Epub 2006 Sep 22.

Identification of CT521 as a frequent target of Th1 cells in patients with urogenital Chlamydia trachomatis infection.

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  • 1Chlamydia Research, Department of Infectious Disease Immunology, Statens Serum Institute, DK-2300 Copenhagen S, Denmark. Aol@ssi.dk

Abstract

BACKGROUND:

The human immune response to a Chlamydia trachomatis serovar D lysate was investigated in patients with urogenital C. trachomatis infection, to identify novel T cell targets.

METHODS:

A C. trachomatis lysate was fractionated on the basis of molecular mass, and each fraction was used to stimulate peripheral-blood mononuclear cells from patients with C. trachomatis infection. In frequently recognized fractions, proteins were identified by mass spectrometry, recombinantly expressed, and tested for T cell recognition.

RESULTS:

T cell recognition of the fractions was highly heterogeneous in patients with C. trachomatis infection (n=16). Four patients exhibited responses that were strongly targeted to antigens of 16-20-kDa molecular mass. Three proteins were identified in this fraction: CT043, CT511, and CT521. The T cell response to the individual recombinant proteins were investigated, and CT521 was found to induce the highest level of interferon (IFN)- gamma. The recognition of CT521 was investigated in a larger study population (n=41), and a positive IFN-gamma response was measured in 83% of the patients. Several T cell epitopes were identified in CT521; in particular, peptide 5 in the central part of the protein was frequently recognized by T cells (63%).

CONCLUSION:

We have identified a novel C. trachomatis antigen, CT521, that is frequently recognized in patients with urogenital C. trachomatis infection.

PMID:
17041852
[PubMed - indexed for MEDLINE]
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