Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Apr 15;849(1-2):154-62. Epub 2006 Oct 10.

Current methods for phosphoprotein isolation and enrichment.

Author information

  • 1Global Protein Science and Supply, AstraZeneca R&D Södertälje, SE-15185 Södertälje, Sweden. Stefan.Schmidt@astrazeneca.com

Abstract

The phosphorylation of proteins is a central paradigm of signal transduction. The substitution of neutral hydroxyl groups of serine, threonine and tyrosine with a negatively charged phosphate group alters the physicochemical and immunogenic properties of the protein, which then can be used to isolate these isoforms. In the last decades several different techniques were applied, attempting to selectively enrich protein populations with this post-translational modification. This review aims to give an overview on the arsenal of available methods to extract phosphoproteins focusing on chromatographic approaches.

PMID:
17035107
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk