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Ann Biomed Eng. 2006 Nov;34(11):1767-77. Epub 2006 Oct 10.

Optimisation of biochemical condition and substrates in vitro for tissue engineering of ligament.

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  • 1Cell and Tissue Engineering and Mechanics group, LEMTA-UMR CNRS 7563, IFR 111-Bioengineering, UHP Nancy, Vandoeuvre-lès-Nancy, 54500, France.


In this work, we analysed the effect of growth factors on in vitro cell proliferation and collagens synthesis by fibroblasts cultured for 72 h on different substrates (silicon sheet with or without 1% gelatin, and glass as control surface) for ligament tissue engineering. A human fibroblast cell line (CRL-2703) was used. The synthesis of type I and type III collagens were evaluated qualitatively and quantitatively by RT-PCR and confocal microscopy, respectively. Cell proliferation was evaluated by two methods: (1) MTT assay (2) cell cycle analysis. It was found that PDGF-AB stimulate the proliferation of fibroblast cultured on gelatin coated silicon sheet in dose dependant manner with a maximum effect at 10 ng ml(-1). The exogenous TGF-beta1 induced the expression of type I and type III collagens in a dose and substrate-dependant manner. We deduce from this work that biochemical conditions and substrates have an important impact for optimisation of the tissue neo synthesis.

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