Abstract
The sel-6 gene was previously identified in a screen for suppressors of the egg-laying defect associated with hypermorphic alleles of lin-12 (Tax et al. 1997). Here we show that sel-6 and two other previously defined genes, mal-2 and emb-4, are the same gene, now called "emb-4." We perform a genetic and molecular characterization of emb-4 and show that it functions cell autonomously as a positive regulator of lin-12 activity. Viable alleles identified as suppressors of lin-12 are partial loss-of-function mutations, whereas the null phenotype encompasses a range of lethal terminal phenotypes that apparently are not related to loss of lin-12/Notch signaling. emb-4 encodes a large nuclearly localized protein containing a predicted ATPase domain and has apparent orthologs in fission yeast, plants, and animals.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Adenosine Triphosphatases / metabolism*
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Animals
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Animals, Genetically Modified
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Caenorhabditis elegans / genetics
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Caenorhabditis elegans / growth & development
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Caenorhabditis elegans / metabolism*
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Caenorhabditis elegans Proteins / genetics
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Caenorhabditis elegans Proteins / metabolism*
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Embryo, Nonmammalian / cytology
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Embryo, Nonmammalian / metabolism
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Female
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Gene Expression Regulation*
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Germ Cells / physiology
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Mutation
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Phenotype
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Phylogeny
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Polymerase Chain Reaction
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Receptors, Notch
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Subcellular Fractions
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Suppression, Genetic
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Vulva / cytology
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Vulva / metabolism
Substances
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Caenorhabditis elegans Proteins
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Lin-12 protein, C elegans
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Membrane Proteins
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Receptors, Notch
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Adenosine Triphosphatases