Appl Environ Microbiol. 2006 Oct;72(10):6833-6.

Establishment of a gene expression system in Ochrobactrum anthropi.

Seleem MN, Ali M, Boyle SM, Mukhopadhyay B, Witonsky SG, Schurig GG, Sriranganathan N.

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Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic and State University, Blacksburg, VA 24060, USA.

Abstract

Genetic studies of Ochrobactrum anthropi are hindered by the lack of a suitable gene expression system. We constructed a set of vectors containing several promoters and a His tag fusion in the N terminus to facilitate protein detection and purification. The new vectors should significantly enhance the genetic manipulation and characterization of O. anthropi.

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PMCID:: PMC1610320

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