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Nat Methods. 2006 Nov;3(11):891-3. Epub 2006 Oct 1.

Nonblinking and long-lasting single-molecule fluorescence imaging.

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  • 1Department of Physics, Emory University, Atlanta, Georgia 30322, USA. irasnik@physics.emory.edu

Abstract

Photobleaching and blinking of fluorophores pose fundamental limitations on the information content of single-molecule fluorescence measurements. Photoinduced blinking of Cy5 has hampered many previous investigations using this popular fluorophore. Here we show that Trolox in combination with the enzymatic oxygen-scavenging system eliminates Cy5 blinking, dramatically reduces photobleaching and improves the signal linearity at high excitation rates, significantly extending the applicability of single-molecule fluorescence techniques.

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