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Nucleic Acids Res. 2006;34(18):e125. Epub 2006 Sep 29.

3D reconstruction and comparison of shapes of DNA minicircles observed by cryo-electron microscopy.

Author information

  • 1Laboratory for Computation and Visualization in Mathematics and Mechanics, EPFL FSB IMB, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland. arnaud.amzallag@epfl.ch

Abstract

We use cryo-electron microscopy to compare 3D shapes of 158 bp long DNA minicircles that differ only in the sequence within an 18 bp block containing either a TATA box or a catabolite activator protein binding site. We present a sorting algorithm that correlates the reconstructed shapes and groups them into distinct categories. We conclude that the presence of the TATA box sequence, which is believed to be easily bent, does not significantly affect the observed shapes.

PMID:
17012274
[PubMed - indexed for MEDLINE]
PMCID:
PMC1635295
Free PMC Article
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