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J Biochem Biophys Methods. 2007 Apr 10;70(3):499-501. Epub 2006 Aug 30.

Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances.

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  • 1Department of Microbiology, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima, 960-1295, Japan.

Abstract

To simplify the molecular detection of micro-organisms, we evaluated the tolerance of loop-mediated isothermal amplification (LAMP) to a culture medium and some biological substances. The sensitivity of LAMP was less affected by the various components of the clinical samples than was polymerase chain reaction (PCR); therefore, DNA purification from samples could be omitted.

PMID:
17011631
[PubMed - indexed for MEDLINE]
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