Abstract
The influence of a host's diet on Butyrivibrio and Pseudobutyrivibrio populations was investigated by competitive PCR. Specific primers were designed and competitive PCRs developed for both groups. Results (from 4 cows with different diets) suggested that high-fiber intake essentially increases the Butyrivibrio amounts in the rumen, whereas high-energy food additives lead to its suppression. The Pseudobutyrivibrio concentration also changed during the experiment but without any significant relation to the host's diet.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animal Feed
-
Animals
-
Butyrates / metabolism*
-
Butyrivibrio / genetics*
-
Butyrivibrio / growth & development
-
Butyrivibrio / isolation & purification
-
Cattle
-
Clostridium / genetics*
-
Clostridium / growth & development
-
Clostridium / isolation & purification
-
Colony Count, Microbial
-
DNA, Bacterial / analysis
-
Dietary Fiber
-
Gram-Negative Aerobic Rods and Cocci / genetics*
-
Gram-Negative Aerobic Rods and Cocci / growth & development
-
Gram-Negative Aerobic Rods and Cocci / isolation & purification
-
Polymerase Chain Reaction / methods
-
RNA, Ribosomal, 16S / classification
-
RNA, Ribosomal, 16S / genetics
-
Rumen / microbiology*
Substances
-
Butyrates
-
DNA, Bacterial
-
Dietary Fiber
-
RNA, Ribosomal, 16S