Format

Send to:

Choose Destination
See comment in PubMed Commons below
J Clin Microbiol. 2006 Dec;44(12):4336-41. Epub 2006 Sep 27.

Specific primers for rapid detection of Microsporum audouinii by PCR in clinical samples.

Author information

  • 1Lab. Micologia, Instituto de Higiene e Medicina Tropical/CREM, Universidade Nova de Lisboa, Portugal.

Abstract

This report describes application of PCR fingerprinting to identify common species of dermatophytes using the microsatellite primers M13, (GACA)4, and (GTG)5. The initial PCR analysis rendered a specific DNA fragment for Microsporum audouinii, which was cloned and sequenced. Based on the sequencing data of this fragment, forward (MA_1F) and reverse (MA_1R) primers were designed and verified by PCR to establish their reliability in the diagnosis of M. audouinii. These primers produced a singular PCR band of 431 bp specific only to strains and isolates of M. audouinii, based on a global test of 182 strains/isolates belonging to 11 species of dermatophytes. These findings indicate these primers are reliable for diagnostic purposes, and we recommend their use in laboratory analysis.

PMID:
17005755
[PubMed - indexed for MEDLINE]
PMCID:
PMC1698423
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk