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Traffic. 2006 Sep;7(9):1283-9.

Detection and quantification of protein-microtubules interactions using green fluorescent protein photoconversion.

Author information

  • 1Biologie du D√©veloppement, CNRS UMR 7622 bat C-5√®me, 9, Quai St Bernard, 75252 Paris Cedex 05, France. sbrunet@snv.jussieu.fr

Abstract

We present an in vitro system to analyze quantitatively the interactions of green fluorescent protein (GFP)-tagged recombinant proteins with microtubules. This method relies on photoconversion of GFP and time-lapse microscopy. Specific interactions can be detected and binding kinetics can be determined rapidly and accurately. This method provides an alternative to classical in vitro microtubule-binding assays to analyze microtubule-associated proteins binding to microtubules. It has the potential to be extended to study interactions of proteins or multi-protein complexes with different biopolymers like actin microfilaments or organelle membranes.

PMID:
17004326
[PubMed - indexed for MEDLINE]
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