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    Biosci Biotechnol Biochem. 2006 Sep;70(9):2154-9.

    Enhanced sequence coverage in tryptic fragment analysis by two-dimensional HPLC/MS using a monolithic silica capillary column.

    Morisaka H, Hata K, Mima J, Tanigawa T, Furuno M, Ishizuka N, Tanaka N, Ueda M.

    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitasshirakawa, Kyoto, Japan.

    The HPLC/MS system, in which a monolithic silica capillary column is directly connected to an electronspray-ionization mass spectrometer, showed superior performance at high mobile phase linear velocity. A two-dimensional (2D) HPLC/MS system was established, using an ion-exchange particle-packed capillary column at the first dimension and a monolithic silica capillary column at the second dimension. In an analysis of tryptic fragments from bovine serum albumin, an 81% sequence coverage, obtained by the 2D-HPLC/MS system, increased by 23% as compared to a 1D-HPLC/MS system. This 2D-HPLC/MS system using a monolithic silica capillary column should be useful for enhancing sequence coverage of tryptic fragments in proteomics.

    PMID: 17003524 [PubMed - indexed for MEDLINE]

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