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    Appl Environ Microbiol. 2006 Nov;72(11):7401-5. Epub 2006 Sep 22.

    Construction of a reporter vector for the analysis of Bifidobacterium longum promoters.

    Source

    Department of Nutrition and Health, Nestlé Research Center, P.O. Box 44, Vers-chez-les-Blanc, 1000 Lausanne 26, Switzerland.

    Abstract

    In order to initiate studies on promoter activities in Bifidobacterium longum and to independently confirm transcriptional data generated by microarray experiments, we have constructed a versatile reporter plasmid based on a B. longum cryptic plasmid and the Escherichia coli gusA gene. The resulting plasmid, pMDY23, has been tested using three B. longum promoters.

    PMID:
    16997985
    [PubMed - indexed for MEDLINE]
    PMCID: PMC1636199
    Free PMC Article

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