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Colloids Surf B Biointerfaces. 2007 Jul 1;58(1):3-7. Epub 2006 Aug 17.

Extinction coefficient of gold nanoparticles with different sizes and different capping ligands.

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  • 1Nanoscience and Nanotechnology Center and Chemistry Department, University of Central Florida, 12424 Research Pkwy, Orlando, FL 32826, USA.


Extinction coefficients of gold nanoparticles with core size ranging from approximately 4 to 40 nm were determined by high resolution transmission electron microscopy analysis and UV-vis absorption spectroscopic measurement. Three different types of gold nanoparticles were prepared and studied: citrate-stabilized nanoparticles in five different sizes; oleylamide-protected gold nanoparticles with a core diameter of 8 nm, and a decanethiol-protected nanoparticle with a diameter of around 4 nm. A linear relationship between the logarithms of extinction coefficients and core diameters of gold particles was found independent of the capping ligands on the particle surface and the solvents used to dissolve the nanoparticles. This linear relation may be used as a calibration curve to determine the concentration or average size of an unknown nanoparticle or nanoparticle-biomolecule conjugate sample.

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