J Immunol Methods. 2006 Oct 20;316(1-2):153-7. Epub 2006 Sep 1.

A novel fluorescent sensitive assay for detection of differential T cell mediated lysis of multiple adherent target cells.

Black AP, Jones L, Ardern-Jones M, Ogg GS.

Source

Cutaneous Immunology Group, MRC Human Immunology Unit, University of Oxford, Weatherall Institute of Molecular Medicine, Oxford OX3 9DS, UK. ablack@hammer.imm.ox.ac.uk

Abstract

There are few studies that have investigated T cell mediated lysis of adherent cells. We have developed a novel, rapid and sensitive fluorescent dye-swap assay that allows efficient detection of adherent target cell lysis. The assay allows simultaneous use of multiple differentially sensitised targets and facilitates concomitant surface or intracellular effector cell phenotypic analysis.

PMID:: 16989854; [PubMed - indexed for MEDLINE]

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