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J Biomed Opt. 2006 Jul-Aug;11(4):044006.

Fourier-domain biophotoacoustic subsurface depth selective amplitude and phase imaging of turbid phantoms and biological tissue.

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  • 1Center for Advanced Diffusion-Wave Technologies University of Toronto Department of Mechanical and Industrial Engineering Toronto, Ontario, M5S 3G8 Canada.


A novel photothermoacoustic imaging modality utilizing a frequency-swept (chirped) intensity-modulated laser source and coherent frequency domain signal processing ("biophotoacoustics") was introduced for noninvasive imaging of biological tissues. The developed frequency-domain imaging system takes advantage of linear frequency modulation waveforms to relate depth of tissue chromophores to the frequency spectrum of the detected acoustic response and of a narrow signal detection bandwidth to improve signal-to-noise ratio (SNR). Application of frequency-domain photothermoacoustic (FD-PTA) imaging was demonstrated using turbid phantoms and ex-vivo specimens of chicken breast with embedded absorbing inclusions simulating tumors.

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