Topology characterization by MALDI-ToF-MS of enzymatically synthesized poly(lactide-co-glycolide)

Saskia Huijser; Bastiaan B P Staal; Juan Huang; Robbert Duchateau; Cor E Koning

doi:10.1021/bm060466v

Topology characterization by MALDI-ToF-MS of enzymatically synthesized poly(lactide-co-glycolide)

Biomacromolecules. 2006 Sep;7(9):2465-9. doi: 10.1021/bm060466v.

Authors

Saskia Huijser¹, Bastiaan B P Staal, Juan Huang, Robbert Duchateau, Cor E Koning

Affiliation

¹ Laboratory of Polymer Chemistry, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands.

PMID: 16961304
DOI: 10.1021/bm060466v

Abstract

Lipase catalyzed copolymerization of the monomers lactide and glycolide by Pseudomonas cepacia employing a molar ratio of 80L/20G has been studied. The copolymers were characterized by MALDI-ToF-MS, DSC, SEC and NMR. MALDI-ToF-MS has successfully been used not only to determine end groups and chemical composition but even the microstructure of the copolymers. We demonstrated that for this lipase catalyzed copolymerization, the main product of the reaction at 100 degrees C was linear homopolymer of lactide while at 130 degrees C the main product was cyclic random copolymer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biocompatible Materials / chemistry*
Biotechnology / methods
Burkholderia cepacia / metabolism
Calorimetry, Differential Scanning
Catalysis
Cations
Lipase / chemistry
Macromolecular Substances / chemistry
Magnetic Resonance Spectroscopy
Mass Spectrometry / methods*
Models, Chemical
Polyglactin 910 / chemistry*
Polymers / chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Temperature

Substances

Biocompatible Materials
Cations
Macromolecular Substances
Polymers
Polyglactin 910
Lipase