Display Settings:


Send to:

Choose Destination
See comment in PubMed Commons below
Methods Mol Biol. 2006;342:57-72.

Mouse embryonic stem cells as a model genetic system to dissect and exploit the RNA interference machinery.

Author information

  • 1The CBR Institute for Biomedical Research and Department of Pathology, Harvard Medical School, Boston, MA, USA.


Conditional gene targeting is often a useful approach to elucidate the in vivo function of a gene. We use this approach to investigate the biological role of the RNA interference (RNAi) pathway in mammals. In addition, the RNAi machinery in mammalian cells can be exploited for gene knock-down experiments. In this chapter, we discuss the variety of experiments that can be performed using genetically engineered embryonic stem (ES) cells. ES cells provide a mammalian genetic system that is physiological, and tractable for mutagenesis and experimentation. This approach is economical and rapid, because it does not require production and breeding of genetically engineered mice.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Icon for Springer
    Loading ...
    Write to the Help Desk