Uronic acid determination by high performance liquid chromatography with postcolumn fluorescence derivatization

Hirotaka Kakita; Hiroshi Kamishima; Kuniyo Inouye

doi:10.1016/j.chroma.2006.08.057

Uronic acid determination by high performance liquid chromatography with postcolumn fluorescence derivatization

J Chromatogr A. 2006 Oct 6;1129(2):296-9. doi: 10.1016/j.chroma.2006.08.057. Epub 2006 Sep 7.

Authors

Hirotaka Kakita¹, Hiroshi Kamishima, Kuniyo Inouye

Affiliation

¹ AIST Shikoku, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu, Kagawa, Japan. h-kakita@aist.go.jp

PMID: 16956616
DOI: 10.1016/j.chroma.2006.08.057

Abstract

To develop a fluorimetric high performance liquid chromatography (HPLC) technique for uronic acid microanalysis, a saline mobile phase and the postcolumn fluorimetric determination were combined. The detection limits of D-glucuronic, D-galacturonic and D-mannuronic acids were 7.19, 23.88 and 7.08 pmol, respectively. The proposed method was successfully applied to uronic acid microanalysis in a polysaccharide hydrolysate and a drink.

MeSH terms

Chromatography, High Pressure Liquid / instrumentation
Chromatography, High Pressure Liquid / methods*
Fluorescence
Glucose / analysis
Glucose / chemistry
Glucuronates / analysis
Glucuronates / chemistry
Hexuronic Acids / analysis
Hexuronic Acids / chemistry
Reproducibility of Results
Uronic Acids / analysis*
Uronic Acids / chemistry

Substances

Glucuronates
Hexuronic Acids
Uronic Acids
galacturonic acid
mannuronic acid
Glucose