Towards many colors in FISH on 3D-preserved interphase nuclei

J Walter; B Joffe; A Bolzer; H Albiez; P A Benedetti; S Müller; M R Speicher; T Cremer; M Cremer; I Solovei

doi:10.1159/000094227

Towards many colors in FISH on 3D-preserved interphase nuclei

Cytogenet Genome Res. 2006;114(3-4):367-78. doi: 10.1159/000094227.

Authors

J Walter¹, B Joffe, A Bolzer, H Albiez, P A Benedetti, S Müller, M R Speicher, T Cremer, M Cremer, I Solovei

Affiliation

¹ Chair of Anthropology and Human Genetics, Department of Biology II, Ludwig-Maximilians-University, Martinsried, Germany.

PMID: 16954680
DOI: 10.1159/000094227

Abstract

The article reviews the existing methods of multicolor FISH on nuclear targets, first of all, interphase chromosomes. FISH proper and image acquisition are considered as two related components of a single process. We discuss (1) M-FISH (combinatorial labeling + deconvolution + wide-field microscopy); (2) multicolor labeling + SIM (structured illumination microscopy); (3) the standard approach to multicolor FISH + CLSM (confocal laser scanning microscopy; one fluorochrome - one color channel); (4) combinatorial labeling + CLSM; (5) non-combinatorial labeling + CLSM + linear unmixing. Two related issues, deconvolution of images acquired with CLSM and correction of data for chromatic Z-shift, are also discussed. All methods are illustrated with practical examples. Finally, several rules of thumb helping to choose an optimal labeling + microscopy combination for the planned experiment are suggested.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Cell Nucleus / genetics
Cell Nucleus / ultrastructure*
Chromosome Painting
Color
Humans
In Situ Hybridization, Fluorescence / methods*
Interphase / genetics*
Microscopy, Fluorescence