Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Cell. 2006 Sep 22;126(6):1189-201. Epub 2006 Aug 31.

Genome-wide high-resolution mapping and functional analysis of DNA methylation in arabidopsis.

Author information

  • 1Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA.

Abstract

Cytosine methylation is important for transposon silencing and epigenetic regulation of endogenous genes, although the extent to which this DNA modification functions to regulate the genome is still unknown. Here we report the first comprehensive DNA methylation map of an entire genome, at 35 base pair resolution, using the flowering plant Arabidopsis thaliana as a model. We find that pericentromeric heterochromatin, repetitive sequences, and regions producing small interfering RNAs are heavily methylated. Unexpectedly, over one-third of expressed genes contain methylation within transcribed regions, whereas only approximately 5% of genes show methylation within promoter regions. Interestingly, genes methylated in transcribed regions are highly expressed and constitutively active, whereas promoter-methylated genes show a greater degree of tissue-specific expression. Whole-genome tiling-array transcriptional profiling of DNA methyltransferase null mutants identified hundreds of genes and intergenic noncoding RNAs with altered expression levels, many of which may be epigenetically controlled by DNA methylation.

Comment in

PMID:
16949657
[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for Faculty of 1000
    Loading ...
    Write to the Help Desk