Human protectin (CD59), an 18-20-kD homologous complement restriction factor, does not restrict perforin-mediated lysis

S Meri; B P Morgan; M Wing; J Jones; A Davies; E Podack; P J Lachmann

doi:10.1084/jem.172.1.367

Human protectin (CD59), an 18-20-kD homologous complement restriction factor, does not restrict perforin-mediated lysis

J Exp Med. 1990 Jul 1;172(1):367-70. doi: 10.1084/jem.172.1.367.

Authors

S Meri¹, B P Morgan, M Wing, J Jones, A Davies, E Podack, P J Lachmann

Affiliation

¹ Molecular Immunopathology Unit, Medical Research Council, Cambridge, United Kingdom.

Abstract

Human protectin (CD59) is an 18-20-kD membrane glycoprotein that restricts lysis of human erythrocytes and leukocytes by homologous complement. By directly incorporating protectin into membranes of heterologous cells we observed that protectin did not prevent perforin-mediated killing, whereas complement killing was effectively restricted. Further, no significant enhancement of cell-mediated killing or target killing by purified perforin was observed with anti-protectin antibodies. Thus, in contrast with complement lysis, restriction of lysis by protectin does not apply to cell-mediated killing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Monoclonal
Antigens, CD / physiology*
Antigens, Differentiation / physiology*
CD59 Antigens
Complement System Proteins / immunology*
Cytotoxicity, Immunologic / immunology
Erythrocytes / immunology
Guinea Pigs
Hemolysis / immunology*
Humans
Killer Cells, Natural / immunology
Membrane Glycoproteins / physiology*
Membrane Proteins / immunology*
Mice
Perforin
Pore Forming Cytotoxic Proteins
Rabbits

Substances

Antibodies, Monoclonal
Antigens, CD
Antigens, Differentiation
CD59 Antigens
Membrane Glycoproteins
Membrane Proteins
Pore Forming Cytotoxic Proteins
Perforin
Complement System Proteins

Grants and funding

Wellcome Trust/United Kingdom