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Nat Methods. 2006 Sep;3(9):715-9.

Genome-scale loss-of-function screening with a lentiviral RNAi library.

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  • 1Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA.


The discovery that RNA interference (RNAi) is functional in mammalian cells led us to form The RNAi Consortium (TRC) with the goal of enabling large-scale loss-of-function screens through the development of genome-scale RNAi libraries and methodologies for their use. These resources form the basis of a loss-of-function screening platform created at the Broad Institute. Our human and mouse libraries currently contain >135,000 lentiviral clones targeting 27,000 genes. Initial screening efforts have demonstrated that these libraries and methods are practical and powerful tools for high-throughput lentivirus RNAi screens.

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