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Appl Biochem Biotechnol. 2006 Spring;129-132:278-88.

Effect of inhibitors released during steam-explosion pretreatment of barley straw on enzymatic hydrolysis.

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  • 1Renewable Energies Department-CIEMAT, Avenida Complutense, 22 28040-Madrid, Spain.


The influence of the liquid fraction (prehydrolysate) generated during steam-explosion pretreatment (210 degrees C, 15 min) of barley straw on the enzymatic hydrolysis was determined. Prehydrolysate was analyzed for degradation compounds and sugars' content and used as a medium for enzymatic hydrolysis tests after pH adjusting to 4.8. Our results show that the presence of the compounds contained in the prehydrolysate strongly affects the hydrolysis step (a 25% decrease in cellulose conversion compared with control). Sugars are shown to be more potent inhibitors of enzymatic hydrolysis than degradation products.

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