Warning: The NCBI web site requires JavaScript to function. more...

My NCBI Sign In

Virology. 2006 Oct 25;354(2):299-315. Epub 2006 Aug 9.

A DNA prime-oral Listeria boost vaccine in rhesus macaques induces a SIV-specific CD8 T cell mucosal response characterized by high levels of alpha4beta7 integrin and an effector memory phenotype.

Neeson P, Boyer J, Kumar S, Lewis MG, Mattias L, Veazey R, Weiner D, Paterson Y.

Source

Microbiology Department, University of Pennsylvania, 323 Johnson Building, 3610 Hamilton Walk, Philadelphia, PA 19104, USA.

Abstract

In this study in Rhesus macaques, we tested whether IL-12 or IL-15 in a DNA prime-oral Listeria boost amplifies the SIV-Gag-specific CD8 mucosal response. SIV-specific CD8 T cells were demonstrated in the peripheral blood (PB) in all test vaccine groups, but not the control group. SIV-Gag-specific CD8 T cells in the PB expressed alpha4beta7 integrin, the gut-homing receptor; a minor subset co-express alphaEbeta7 integrin. SIV-Gag-specific CD8 T cells were also detected in the gut tissue, intraepithelial (IEL) and lamina propria lymphocytes (LPL) of the duodenum and ileum. These cells were characterized by high levels of beta7 integrin expression and a predominance of the effector memory phenotype. Neither Il-12 nor IL-15 amplified the frequency of SIV-specific CD8 T cells in the gut. Thus, the DNA prime-oral Listeria boost strategy induced a mucosal SIV-Gag-specific CD8 T cell response characterized by expression of the alpha4beta7 integrin gut-homing receptor.

PMID:: 16904153; [PubMed - indexed for MEDLINE]
PMCID: PMC1635491

Free PMC Article

Images from this publication.See all images (12) Free text

Suppl Figure 3

Suppl Figure 1

Suppl Figure 2

LinkOut - more resources

Full Text Sources

Supplemental Content

Click here to read

Click here to read

Related citations

Mucosal priming of simian immunodeficiency virus-specific cytotoxic T-lymphocyte responses in rhesus macaques by the Salmonella type III secretion antigen delivery system. [J Virol. 2003]
Mucosal priming of simian immunodeficiency virus-specific cytotoxic T-lymphocyte responses in rhesus macaques by the Salmonella type III secretion antigen delivery system.
Evans DT, Chen LM, Gillis J, Lin KC, Harty B, Mazzara GP, Donis RO, Mansfield KG, Lifson JD, Desrosiers RC, et al. J Virol. 2003 Feb; 77(4):2400-9.
Heterogeneity of the simian immunodeficiency virus (SIV) specific CD8(+) T-cell response in mucosal tissues during SIV primary infection. [Microbes Infect. 2003]
Heterogeneity of the simian immunodeficiency virus (SIV) specific CD8(+) T-cell response in mucosal tissues during SIV primary infection.
Vingert BC, Le Grand R, Venet A. Microbes Infect. 2003 Jul; 5(9):757-67.
Therapeutic vaccination with simian immunodeficiency virus (SIV)-DNA + IL-12 or IL-15 induces distinct CD8 memory subsets in SIV-infected macaques. [J Immunol. 2008]
Therapeutic vaccination with simian immunodeficiency virus (SIV)-DNA + IL-12 or IL-15 induces distinct CD8 memory subsets in SIV-infected macaques.
Halwani R, Boyer JD, Yassine-Diab B, Haddad EK, Robinson TM, Kumar S, Parkinson R, Wu L, Sidhu MK, Phillipson-Weiner R, et al. J Immunol. 2008 Jun 15; 180(12):7969-79.
Review Use of nonhuman primate models to develop mucosal AIDS vaccines. [Curr HIV/AIDS Rep. 2010]
Review Use of nonhuman primate models to develop mucosal AIDS vaccines.
Genescà M, Miller CJ. Curr HIV/AIDS Rep. 2010 Feb; 7(1):19-27.
Review Mucosal HIV vaccines: where are we now? [Curr HIV Res. 2004]
Review Mucosal HIV vaccines: where are we now?
Stevceva L, Strober W. Curr HIV Res. 2004 Jan; 2(1):1-10.

See reviews... See all...

Cited by 7 PubMed Central articles

Distinct Humoral and Cellular Immunity Induced by Alternating Prime-boost Vaccination Using Plasmid DNA and Live Viral Vector Vaccines Expressing the E Protein of Dengue Virus Type 2. [Immune Netw. 2011]
Distinct Humoral and Cellular Immunity Induced by Alternating Prime-boost Vaccination Using Plasmid DNA and Live Viral Vector Vaccines Expressing the E Protein of Dengue Virus Type 2.
George JA, Eo SK. Immune Netw. 2011 Oct; 11(5):268-80. Epub 2011 Oct 31.
Durable mucosal simian immunodeficiency virus-specific effector memory T lymphocyte responses elicited by recombinant adenovirus vectors in rhesus monkeys. [J Virol. 2011]
Durable mucosal simian immunodeficiency virus-specific effector memory T lymphocyte responses elicited by recombinant adenovirus vectors in rhesus monkeys.
Li H, Liu J, Carville A, Mansfield KG, Lynch D, Barouch DH. J Virol. 2011 Nov; 85(21):11007-15. Epub 2011 Sep 14.
Long-lasting humoral and cellular immune responses and mucosal dissemination after intramuscular DNA immunization. [Vaccine. 2010]
Long-lasting humoral and cellular immune responses and mucosal dissemination after intramuscular DNA immunization.
Patel V, Valentin A, Kulkarni V, Rosati M, Bergamaschi C, Jalah R, Alicea C, Minang JT, Trivett MT, Ohlen C, et al. Vaccine. 2010 Jul 5; 28(30):4827-36. Epub 2010 May 6.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

A DNA prime-oral Listeria boost vaccine in rhesus macaques induces a SIV-specifi...
A DNA prime-oral Listeria boost vaccine in rhesus macaques induces a SIV-specific CD8 T cell mucosal response characterized by high levels of alpha4beta7 integrin and an effector memory phenotype.
Virology. 2006 Oct 25 ;354(2):299-315. Epub 2006 Aug 9 .

PubMed
Obesity and lower urinary tract function in women: effect of surgically induced ...
Obesity and lower urinary tract function in women: effect of surgically induced weight loss.
Am J Obstet Gynecol. 1992 Aug ;167(2):392-7; discussion 397-9.

PubMed
A report--chronic fatigue syndrome: guidelines for research.
A report--chronic fatigue syndrome: guidelines for research.
J R Soc Med. 1991 Feb; 84(2): 118-121.

PMC
Quantitative analysis of EGFRvIII cellular signaling networks reveals a combinat...
Quantitative analysis of EGFRvIII cellular signaling networks reveals a combinatorial therapeutic strategy for glioblastoma.
Proc Natl Acad Sci U S A. 2007 Jul 31 ;104(31):12867-72. Epub 2007 Jul 23 .

PubMed
Stories or statistics? Farmers' attitudes toward messages in an agricultural saf...
Stories or statistics? Farmers' attitudes toward messages in an agricultural safety campaign.
J Agric Saf Health. 2002 May ;8(2):225-39.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...

You are here: NCBI > Literature > PubMed

Write to the Help Desk