Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Genome Biol. 2006;7(8):R71. Epub 2006 Aug 4.

Zebrafish promoter microarrays identify actively transcribed embryonic genes.

Author information

  • 1Wellcome Trust/Cancer Research UK Gurdon Institute and Department of Zoology, Cambridge University, Cambridge, UK. fcw27@cam.ac.uk

Abstract

We have designed a zebrafish genomic microarray to identify DNA-protein interactions in the proximal promoter regions of over 11,000 zebrafish genes. Using these microarrays, together with chromatin immunoprecipitation with an antibody directed against tri-methylated lysine 4 of Histone H3, we demonstrate the feasibility of this method in zebrafish. This approach will allow investigators to determine the genomic binding locations of DNA interacting proteins during development and expedite the assembly of the genetic networks that regulate embryogenesis.

PMID:
16889661
[PubMed - indexed for MEDLINE]
PMCID:
PMC1779600
Free PMC Article

Images from this publication.See all images (6)Free text

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for BioMed Central Icon for PubMed Central
    Loading ...
    Write to the Help Desk