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Microsc Res Tech. 2006 Nov;69(11):891-3.

Evaluation of a femtosecond fiber laser for two-photon fluorescence correlation spectroscopy.

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  • 1Department of Chemistry, University of Kansas, Lawrence, Kansas 66045, USA.

Abstract

This work evaluates a femtosecond fiber laser for use in two-photon fluorescence fluctuation spectroscopy. Fiber lasers present an attractive alternative to Ti:Sapphire systems because of their compact size and portability. Autocorrelation of the second harmonic generation signal from the laser demonstrates that its stability is sufficient for two-photon fluorescence correlation spectroscopy. Fluorescence correlation spectroscopy autocorrelation traces were well fit by a Gaussian-Lorentzian squared model with a beam waist near the diffraction limit for the 810 nm wavelength. A photon counting histogram collected with this system also fit nicely to a single-species model, further demonstrating the quality of the focal shape. The authors conclude that the output from the femtosecond fiber laser is sufficiently stable and has a high enough quality beam shape for fluctuation fluorescence methods, and thus represents an effective, compact, readily portable two-photon excitation source.

(c) 2006 Wiley-Liss, Inc.

PMID:
16886226
[PubMed]
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