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Genome Biol. 2006;7(7):R66.

Analysis of cell-based RNAi screens.

Author information

  • 1Signaling and Functional Genomics, German Cancer Research Center, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany. m.boutros@dkfz.de

Abstract

RNA interference (RNAi) screening is a powerful technology for functional characterization of biological pathways. Interpretation of RNAi screens requires computational and statistical analysis techniques. We describe a method that integrates all steps to generate a scored phenotype list from raw data. It is implemented in an open-source Bioconductor/R package, cellHTS (http://www.dkfz.de/signaling/cellHTS). The method is useful for the analysis and documentation of individual RNAi screens. Moreover, it is a prerequisite for the integration of multiple experiments.

PMID:
16869968
[PubMed - indexed for MEDLINE]
PMCID:
PMC1779553
Free PMC Article

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