Your browser version may not work well with NCBI's Web applications. More information here...
1: Genome Biol. 2006;7(7):R61.Click here to read Click here to read Links

A method for high-throughput gene expression signature analysis.

Peck D, Crawford ED, Ross KN, Stegmaier K, Golub TR, Lamb J.

Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA. dpeck@broad.mit.edu

Genome-wide transcriptional profiling has shown that different biologic states (for instance, disease and response to pharmacologic manipulation) can be recognized by the expression pattern of relatively small numbers of genes. However, the lack of a practical and cost-effective technology for detection of these gene expression 'signatures' in large numbers of samples has severely limited their exploitation in important medical and pharmaceutical discovery applications. Here, we describe a solution based on the combination of ligation-mediated amplification with an optically addressed microsphere and flow cytometric detection system.

PMID: 16859521 [PubMed - indexed for MEDLINE]

PMCID: PMC1779561