Abstract
We investigated the putative toxins of Philodryas olfersii (Colubridae), a representative of a family of snakes neglected in venom studies despite their growing medical importance. Transcriptomic data of the venom gland complemented by proteomic analysis of the gland secretion revealed the presence of major toxin classes from the Viperidae family, including serine proteases, metalloproteases, C-type lectins, Crisps, and a C-type natriuretic peptide (CNP). Interestingly, the phylogenetic analysis of the CNP precursor showed it as a linker between two related precursors found in Viperidae and Elapidae snakes. We suggest that these precursors constitute a monophyletic group derived from the vertebrate CNPs.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Colubridae / classification*
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Colubridae / genetics
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Colubridae / metabolism
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Elapidae / classification
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Evolution, Molecular
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Expressed Sequence Tags / chemistry
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Female
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Lectins, C-Type / analysis
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Lectins, C-Type / chemistry
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Lectins, C-Type / genetics
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Male
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Metalloproteases / analysis
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Metalloproteases / chemistry
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Metalloproteases / genetics
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Molecular Sequence Data
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Natriuretic Peptides / chemistry
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Natriuretic Peptides / classification
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Natriuretic Peptides / genetics
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Oligopeptides / chemistry
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Oligopeptides / classification
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Oligopeptides / genetics
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Phylogeny
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Protein Precursors / chemistry
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Protein Precursors / classification
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Protein Precursors / genetics
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Proteome / chemistry
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Proteome / classification
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Proteome / genetics
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Sequence Alignment
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Serine Endopeptidases / analysis
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Serine Endopeptidases / chemistry
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Serine Endopeptidases / genetics
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Snake Venoms / chemistry
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Snake Venoms / classification*
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Snake Venoms / genetics
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Transcription, Genetic
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Viperidae / classification
Substances
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Lectins, C-Type
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Natriuretic Peptides
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Oligopeptides
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Protein Precursors
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Proteome
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Snake Venoms
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bradykinin potentiating factors
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Metalloproteases
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Serine Endopeptidases