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Human glandular Kallikrein genes: genetic and physical mapping of the KLK1 locus using a highly polymorphic microsatellite PCR marker.
Department of Cytogenetics and Molecular Genetics, Adelaide Children's Hospital, Australia.
We describe a highly polymorphic microsatellite repeat sequence, KLK1 AC, which is located 3' to the human glandular kallikrein gene (KLK1) at 19q13.3-13.4. A multiplex PCR was developed to simultaneously genotype the KLK1 AC repeat length polymorphism and a similar repeat at the adjacent APOC2 locus at 19q13.2. Genotypes from these two loci in the 40 large kindred pedigrees from the Centre d'Etude du Polymorphisme Humain were used in conjunction with the background genetic map to establish a multipoint linkage map. The KLK1 locus was also localized physically using somatic cell hybrid DNA templates for polymerase chain reaction analysis. Both genetic and physical mapping studies are consistent with the assignment cen-APOC2-KLK1-D19522-qter. The linkage map places KLK1 approximately 10 cM distal to APOC2. These markers therefore flank the myotonic dystrophy gene and may be useful for diagnosis.
PMID: 1684954 [PubMed - indexed for MEDLINE]
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Cited by 3 PubMed Central articles
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Evidence for human meiotic recombination interference obtained through construction of a short tandem repeat-polymorphism linkage map of chromosome 19.
Weber JL, Wang Z, Hansen K, Stephenson M, Kappel C, Salzman S, Wilkie PJ, Keats B, Dracopoli NC, Brandriff BF.
Am J Hum Genet. 1993 Nov; 53(5):1079-95.
[Am J Hum Genet. 1993]
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Fragile X syndrome: genetic localisation by linkage mapping of two microsatellite repeats FRAXAC1 and FRAXAC2 which immediately flank the fragile site.
Richards RI, Holman K, Kozman H, Kremer E, Lynch M, Pritchard M, Yu S, Mulley J, Sutherland GR.
J Med Genet. 1991 Dec; 28(12):818-23.
[J Med Genet. 1991]
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New nucleotide sequence data on the EMBL File Server.
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Nucleic Acids Res. 1992 Mar 25; 20(6):1435-48.
[Nucleic Acids Res. 1992]