Methods Mol Biol. 2006;329:59-79.

Derivation, maintenance, and induction of the differentiation in vitro of equine embryonic stem cells.

Saito S, Sawai K, Minamihashi A, Ugai H, Murata T, Yokoyama KK.

Source

Saito Laboratory of Cell Technology, Kataoka, Yaita, Techigi, Japan.

Abstract

We describe here the isolation and maintenance of pluripotent embryonic stem (ES) cells from equine blastocysts that have been frozen and thawed. Equine ES cells appear to maintain a normal diploid karyotype in culture. These cells express markers that are characteristic of mouse ES cells, namely, alkaline phosphatase, stage-specific-embryonic antigen 1, STAT3, and Oct4. We also describe protocols for the induction of differentiation in vitro to neural precursor cells in the presence of basic fibroblast growth factor (bFGF), epidermal growth factor, and platelet-derived growth factor and to hematopoietic and endothelial cell lineages in the presence of bFGF, stem cell factor, and oncostatin M. Equine ES cells provide a powerful tool for gene targeting and the generation of transgenic clonal offspring.

PMID:: 16845984; [PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Springer

Other Literature Sources

COS Scholar Universe

Supplemental Content

Save items

Related citations in PubMed

Isolation of embryonic stem-like cells from equine blastocysts and their differentiation in vitro. [FEBS Lett. 2002]
Isolation of embryonic stem-like cells from equine blastocysts and their differentiation in vitro.
Saito S, Ugai H, Sawai K, Yamamoto Y, Minamihashi A, Kurosaka K, Kobayashi Y, Murata T, Obata Y, Yokoyama K. FEBS Lett. 2002 Nov 20; 531(3):389-96.
Derivation, maintenance, and characterization of rat embryonic stem cells in vitro. [Methods Mol Biol. 2006]
Derivation, maintenance, and characterization of rat embryonic stem cells in vitro.
Schulze M, Ungefroren H, Bader M, Fändrich F. Methods Mol Biol. 2006; 329:45-58.
Generation and characterization of monkey embryonic stem cells. [Methods Mol Biol. 2006]
Generation and characterization of monkey embryonic stem cells.
Suemori H, Nakatsuji N. Methods Mol Biol. 2006; 329:81-9.
Review Animal embryonic stem (ES) cells: self-renewal, pluripotency, transgenesis and nuclear transfer. [Hum Cell. 2004]
Review Animal embryonic stem (ES) cells: self-renewal, pluripotency, transgenesis and nuclear transfer.
Saito S, Liu B, Yokoyama K. Hum Cell. 2004 Sep; 17(3):107-15.
Review Derivation and induction of the differentiation of animal ES cells as well as human pluripotent stem cells derived from fetal membrane. [Hum Cell. 2005]
Review Derivation and induction of the differentiation of animal ES cells as well as human pluripotent stem cells derived from fetal membrane.
Saito S, Yokoyama K, Tamagawa T, Ishiwata I. Hum Cell. 2005 Sep; 18(3):135-41.

See reviews... See all...

Cited by 2 PubMed Central articles

Horse bone marrow mesenchymal stem cells express embryo stem cell markers and show the ability for tenogenic differentiation by in vitro exposure to BMP-12. [BMC Cell Biol. 2009]
Horse bone marrow mesenchymal stem cells express embryo stem cell markers and show the ability for tenogenic differentiation by in vitro exposure to BMP-12.
Violini S, Ramelli P, Pisani LF, Gorni C, Mariani P. BMC Cell Biol. 2009 Apr 22; 10:29. Epub 2009 Apr 22.
Review Concepts for the clinical use of stem cells in equine medicine. [Can Vet J. 2008]
Review Concepts for the clinical use of stem cells in equine medicine.
Koch TG, Berg LC, Betts DH. Can Vet J. 2008 Oct; 49(10):1009-17.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Derivation, maintenance, and induction of the differentiation in vitro of equine...
Derivation, maintenance, and induction of the differentiation in vitro of equine embryonic stem cells.
Methods Mol Biol. 2006 ;329:59-79.

PubMed
Diagnosing occupational asthma: insight from induced sputum.
Diagnosing occupational asthma: insight from induced sputum.
Can J Physiol Pharmacol. 2006 Jan ;84(1):1-4.

PubMed
Biomaterial-based notch signaling for the differentiation of hematopoietic stem ...
Biomaterial-based notch signaling for the differentiation of hematopoietic stem cells into T cells.
J Biomed Mater Res A. 2006 Dec 1 ;79(3):689-97.

PubMed
Multipoint linkage analysis in X-linked ocular albinism of the Nettleship-Falls ...
Multipoint linkage analysis in X-linked ocular albinism of the Nettleship-Falls type.
Hum Genet. 1991 Dec ;88(2):162-6.

PubMed
Cytokine expression patterns associated with systemic adverse events following s...
Cytokine expression patterns associated with systemic adverse events following smallpox immunization.
J Infect Dis. 2006 Aug 15 ;194(4):444-53. Epub 2006 Jul 13 .

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Derivation, maintenance, and induction of the differentiation in vitro of equine embryonic stem cells.

Source

Abstract

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Supplemental Content

Save items

Related citations in PubMed

Cited by 2 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information