Curr Opin Biotechnol. 2006 Aug;17(4):359-66. Epub 2006 Jul 12.

Cloning technologies for protein expression and purification.

Source

Protein Expression Laboratory, Research Technology Program, SAIC-Frederick, Inc, NCI-Frederick, Frederick, MD 21702, USA. hartley@ncifcrf.gov

Abstract

Detailed knowledge of the biochemistry and structure of individual proteins is fundamental to biomedical research. To further our understanding, however, proteins need to be purified in sufficient quantities, usually from recombinant sources. Although the sequences of genomes are now produced in automated factories purified proteins are not, because their behavior is much more variable. The construction of plasmids and viruses to overexpress proteins for their purification is often tedious. Alternatives to traditional methods that are faster, easier and more flexible are needed and are becoming available.

PMID:: 16839756; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

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Recombinant Proteins

Grant Support

N01-CO-12400/CO/NCI NIH HHS/United States

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Cloning technologies for protein expression and purification.
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