J Neural Transm Gen Sect. 1991;86(2):151-7.

Electrochemical detection of human brain transmitter amino acids by high-performance liquid chromatography of stable o-phthalaldehyde-sulphite derivatives.

Pearson SJ, Czudek C, Mercer K, Reynolds GP.

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Department of Biomedical Science, University of Sheffield, United Kingdom.

Abstract

A simple, sensitive, reliable and reproducible isocratic HPLC technique for the measurement of OPA/sulphite derivatives of human brain amino acid neurotransmitters is described. This employs a sample preparation that is also compatible with the concurrent determination of monoamines and their metabolites on a separate HPLC system. The method has been applied to the determination of GABA and glutamate in brain tissue taken post-mortem from patients with Huntington's disease and control subjects.

PMID:: 1683240; [PubMed - indexed for MEDLINE]

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Research Support, Non-U.S. Gov't

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Wellcome Trust/United Kingdom

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